| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Rhodopsin;Opsin-2;RHO;OPN2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Rhodopsin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-RHO antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AADI-18; isotype Rabbit IgG; reactivity: Human,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Rhodopsin Rabbit Monoclonal Antibody catalog # M00083. Tested in WB, IHC applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: RHO (Rhodopsin).
- Antibody format: Monoclonal; clone AADI-18; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
RHO (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal. Reported cellular localization context: Membrane ; Multi-pass membrane protein . Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia. Tissue expression notes (as provided): Rod shaped photoreceptor cells which mediates vision in dim light.
Research relevance and current trends
- Research context keywords from the source record include: G Protein Signaling,Neuroscience,Sensory System,Signal Transduction,Signaling Pathway,Visual System.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate RHO antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect RHO expression by Western blot in cell or tissue lysates, Detect RHO in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 46 kDa; calculated MW: 38893 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 46 kDa
- Cellular localization (provided): Membrane ; Multi-pass membrane protein . Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
- Tissue details (provided): Rod shaped photoreceptor cells which mediates vision in dim light.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
