| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rhodopsin;Opsin-2;RHO;OPN2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Rhodopsin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AADI-18 is a rabbit monoclonal antibody that recognizes rhodopsin in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 38.9 kDa, and the supplier reports an observed band at about 39 kDa.
Rhodopsin (RHO) is the light-sensing G protein-coupled receptor of rod photoreceptors. It carries 11-cis-retinal, which isomerizes to all-trans-retinal when it absorbs a photon, activating the G protein transducin and starting the phototransduction cascade for dim-light vision. Rhodopsin mutations are a leading cause of autosomal dominant retinitis pigmentosa, and anti-rhodopsin staining is a standard rod marker.
| Target | Rhodopsin (gene RHO; UniProt P08100, human) |
|---|---|
| Clone | AADI-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.9 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): rat eyeball.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human rhodopsin.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places rhodopsin in the photoreceptor outer segment membrane and membrane. Tissue expression noted by UniProt (human): Rod shaped photoreceptor cells which mediate vision in dim light. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
