| Field | Specification |
|---|---|
| Target | |
| Alternative names | Receptor-interacting serine/threonine-protein kinase 1;2.7.11.1;Cell death protein RIP;Receptor-interacting protein 1;RIP-1;Serine/threonine-protein kinase RIP;RIPK1;RIP, RIP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RIP recombinant protein (Position: K316-N671). Human RIP shares 65% amino acid (aa) sequence identity with mouse RIP. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects receptor-interacting serine/threonine-protein kinase 1 (gene RIPK1) in human samples and is validated for Western blot. The predicted molecular weight is 75.9 kDa, and the supplier reports an observed band at about 76 kDa.
RIPK1 is a serine/threonine kinase that acts as a switch in TNF receptor signaling. As a scaffold in the TNF receptor complex, it supports NF-κB activation and cell survival, while its kinase activity can instead drive apoptosis or, with RIPK3 and MLKL, necroptosis. RIPK1 is a major focus in research on inflammation, cell death and inflammatory disease.
| Target | Receptor-interacting serine/threonine-protein kinase 1 (gene RIPK1; UniProt Q13546, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 75.9 kDa |
| Observed MW | 76 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human 22RV1 cells, human MCF-7 cells, human HeLa cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human RIP fragment (Lys316–Asn671), expressed in E. coli. Sequence identity with the mouse ortholog: 65%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places receptor-interacting serine/threonine-protein kinase 1 in the cytoplasm and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Miao-Miao Jia et al. (2020) Jujuboside B promotes the death of acute leukemia cell in a RIPK1/RIPK3/MLKL pathway-dependent manner. European Journal of Pharmacology. 10.1016/j.ejphar.2020.173041
Yong-Feng Chen et al. (2014) The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice. International Journal of Clinical and Experimental Pathology. PMID: 25674205
