| Field | Specification |
|---|---|
| Target | |
| Alternative names | RecQ-mediated genome instability protein 2; hRMI2; BLM-associated protein of 18 kDa; BLAP18; RMI2; C16orf75 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RMI2, which shares 96.3% and 92.6% amino acid (aa) sequence identity with mouse and rat RMI2, respectively. |
| Molecular weight | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects recQ-mediated genome instability protein 2 (gene RMI2) in human, mouse and rat samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 15.9 kDa, and the supplier reports an observed band at about 19 kDa.
RMI2 is a small OB-fold protein that joins RMI1, BLM helicase and topoisomerase IIIα in the BTR (BLM–TOP3A–RMI) complex. This complex resolves homologous recombination intermediates such as double Holliday junctions without crossovers, and RMI2 is needed for BLM stability and its recruitment to chromatin, linking it to genome stability research.
| Target | RecQ-mediated genome instability protein 2 (gene RMI2; UniProt Q96E14, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 15.9 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human HeLa cells, human T-47D cells, human HepG2 cells, human K562 cells, rat thymus tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, rat liver tissue, human mammary cancer tissue, mouse liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Synthetic peptide from the C-terminal region of human RMI2. Sequence identity with the mouse and rat orthologs: 96.3% and 92.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places recQ-mediated genome instability protein 2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fei Wang et al. (2024) Glabridin inhibits proliferation and migration in hepatocellular carcinoma by regulating multi-targets. Journal of Ethnopharmacology. 10.1016/j.jep.2024.119022
