| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Rho-related GTP-binding protein Rho6;Rho family GTPase 1;Rnd1;RND1;RHO6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human RND1 recombinant protein (Position: K105-C229). Human RND1 shares 95.2% amino acid (aa) sequence identity with mouse RND1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of RND1 (AP-2 complex subunit mu) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-RND1 Antibody Picoband® catalog # A06347. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human RND1 recombinant protein (Position: K105-C229). Human RND1 shares 95.2% amino acid (aa) sequence identity with mouse RND1. (reported region: K105-C229).
- Molecular weight context: reported MW: 26 kDa; calculated MW: 26056 MW
- Reactivity: Human,Mouse
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
AP-2 complex subunit mu; Rho-related GTP-binding protein Rho6. Rnd1 is a small (~21 kDa) signaling G protein (to be specific, a GTPase), and is a member of the Rndsubgroup of the Rho family of GTPases. Members of this family regulate the organization of the actin cytoskeleton in response to extracellular growth factors. A similar protein in rat interacts with a microtubule regulator to control axon extension. The RND1 gene is mapped to chromosome 12q12-q13. Functional note: Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. . Reported localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton . Expression/tissue context: Mostly expressed in brain and liver.
Research relevance and current trends
- Actin Assembly: Researchers commonly examine how RND1 (AP-2 complex subunit mu) relates to this theme using model systems and orthogonal readouts.
- Actin: Researchers commonly examine how RND1 (AP-2 complex subunit mu) relates to this theme using model systems and orthogonal readouts.
- etc.: Researchers commonly examine how RND1 (AP-2 complex subunit mu) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative RND1 (AP-2 complex subunit mu) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
