Anti-RNF123 Antibody Picoband®

Overview

Anti-RNF123 Antibody Picoband® is an antibody for RNF123 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: RNF123 (ring finger protein 123); UniProt: Q5XPI4
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 149 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-RNF123 Antibody Picoband® catalog # A09642-1.

Biological background

Biological context: Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.

Expression and localization notes: cellular localization: Cytoplasm., tissue context: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines..

Common research applications

• Western blotting (WB): Compare RNF123 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of RNF123 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify RNF123-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: E3 ubiquitin-protein ligase RNF123 is an enzyme that in humans is encoded by the RNF123 gene. It is mapped to 3p21.31. The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm.
• Tissue details: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
• Research category: Cell Biology,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,Ring Finger E3 Ligase,Ubiquitin E3 Enzymes

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.