| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rho-associated protein kinase 1;2.7.11.1;Renal carcinoma antigen NY-REN-35;Rho-associated, coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK-I;p160 ROCK-1;p160ROCK;ROCK1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ROCK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CDE-18 is a rabbit monoclonal antibody that recognizes rho-associated protein kinase 1 (gene ROCK1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 158.2 kDa, and the supplier reports an observed band at about 170 kDa.
ROCK1 (Rho-associated protein kinase 1) is a serine/threonine kinase activated by GTP-bound RhoA. It phosphorylates targets such as myosin light chain, the myosin phosphatase subunit MYPT1 and LIM kinases to drive actomyosin contraction, stress fiber formation and cell migration, and ROCK inhibitors such as Y-27632 are standard research tools.
| Target | Rho-associated protein kinase 1 (gene ROCK1; UniProt Q13464, human) |
|---|---|
| Clone | CDE-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 158.2 kDa |
| Observed MW | 170 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human U2OS cells, human 293T cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human kidney tissue, rat stomach tissue, rat heart tissue, human squamous carcinoma tissue, human esophageal carcinoma tissue, mouse heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide based on the sequence of human ROCK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places rho-associated protein kinase 1 in the cytoplasm, Golgi apparatus membrane, cell projection and cell membrane. Tissue expression noted by UniProt (human): Detected in blood platelets. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongran Zhao et al. (2022) High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway. Investigative Ophthalmology & Visual Science. 10.1167/iovs.63.8.22
Jun Ma et al. (2009) ROCK pathway participates in the processes that 15-hydroxyeicosatetraenoic acid (15-HETE) mediated the pulmonary vascular remodeling induced by hypoxia in rat. Journal of Cellular Physiology. 10.1002/jcp.21923
Li Haojie et al. (2021) Dietary Calcium Alleviates Fluorine-Induced Liver Injury in Rats by Mitochondrial Apoptosis Pathway. Biological Trace Element Research. 10.1007/s12011-021-02641-1
Dongcai Wu et al. (2019) Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2019.108836
Yunbo Chen et al. (2014) β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice. Brain Research. 10.1016/j.brainres.2014.01.005
Xiaowen Huang et al. (2024) Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway. Chemistry & Biodiversity. 10.1002/cbdv.202301383
Jianying Gong et al. (2018) Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling. Medical Science Monitor. 10.12659/MSM.907277
