| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rho-associated protein kinase 2;2.7.11.1;Rho kinase 2;Rho-associated, coiled-coil-containing protein kinase 2;Rho-associated, coiled-coil-containing protein kinase II;ROCK-II;p164 ROCK-2;ROCK2;KIAA0619; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects rho-associated protein kinase 2 (gene ROCK2) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 160.9 kDa, and the supplier reports an observed band at about 161 kDa.
ROCK2 (Rho-associated protein kinase 2) is a RhoA-activated serine/threonine kinase closely related to ROCK1. It controls actomyosin contractility, focal adhesions and cell shape by phosphorylating regulators such as myosin light chain and MYPT1, and it is studied in smooth muscle contraction, fibrosis, neuronal outgrowth and immune regulation.
| Target | Rho-associated protein kinase 2 (gene ROCK2; UniProt O75116, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 160.9 kDa |
| Observed MW | 161 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HeLa cells, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse cardiac muscle tissue, rat brain tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ROCK2 fragment (Glu652–Asp923), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94.9% and 95.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places rho-associated protein kinase 2 in the cytoplasm, cell membrane and nucleus. Tissue expression noted by UniProt (human): Expressed in the brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zheng Juyan et al. (2022) Midazolam Ameliorates Impairment of the Blood–Brain Barrier (BBB) Against LPS. Neurotoxicity Research. 10.1007/s12640-022-00508-4
Fan Shaohua et al. (2020) Glucagon-like peptide 1 reverses myocardial hypertrophy through cAMP/PKA/RhoA/ROCK2 signaling. ACTA Biochimica et Biophysica Sinica. 10.1093/abbs/gmaa038
Feng Qiao et al. (2016) ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension. Experimental and Therapeutic Medicine. 10.3892/etm.2016.3214
