| Field | Specification |
|---|---|
| Target | |
| Alternative names | X-linked retinitis pigmentosa GTPase regulator; RPGR; RP3; XLRP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human RPGR recombinant protein (Position: A13-F338). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes X-linked retinitis pigmentosa GTPase regulator (gene RPGR) in rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 113.4 kDa, and the supplier reports an observed band at about 113 kDa.
RPGR (retinitis pigmentosa GTPase regulator) is a ciliary protein that is especially important in the connecting cilium of photoreceptors. It acts as a guanine nucleotide exchange factor for RAB8A and supports protein trafficking to the outer segment, and mutations in RPGR are the most common cause of X-linked retinitis pigmentosa.
| Target | X-linked retinitis pigmentosa GTPase regulator (gene RPGR; UniProt Q92834, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 113.4 kDa |
| Observed MW | 113 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat kidney tissue, rat brain tissue, rat heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human RPGR fragment (Ala13–Phe338), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places X-linked retinitis pigmentosa GTPase regulator in the Golgi apparatus and cell projection. Tissue expression noted by UniProt (human): Heart, brain, placenta, lung, liver, muscle, kidney, retina, pancreas and fetal retinal pigment epithelium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
