| Field | Specification |
|---|---|
| Target | |
| Alternative names | Large ribosomal subunit protein eL32; 60S ribosomal protein L32; RPL32 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RPL32, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets large ribosomal subunit protein eL32 (gene RPL32) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 15.9 kDa, and the supplier reports an observed band at about 18 kDa.
RPL32 (eL32) is a component of the large 60S ribosomal subunit and contributes to the ribosome's role in protein synthesis. Its transcript is widely used as a reference gene in qPCR studies, and the protein is examined in research on ribosome composition and translation.
| Target | Large ribosomal subunit protein eL32 (gene RPL32; UniProt P62910, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 15.9 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL/ml (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL/ml (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL/ml (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/mL/1×106 cells (Human) |
Samples with a confirmed band (WB): human RT4 cells, human U251 cells, human HepG2 cells, human 293T cells, human HeLa cells, human A431 cells, human A549 cells, human THP-1 cells, rat liver tissue, rat pancreas tissue, rat NRK cells, mouse liver tissue, mouse pancreas tissue, rat stomach tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human breast cancer tissue, human endometrial adenocarcinoma tissue, human esophageal squamous carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with confirmed staining (ICC/IF): human breast cancer tissue.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Synthetic peptide from the C-terminal region of human RPL32. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places large ribosomal subunit protein eL32 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Baiming Huang et al. (2025) Enhanced regenerative and developmental potential of embryonal and stem cell-derived platelets compared to adult platelets. Cell Reports Medicine. 10.1016/j.xcrm.2025.102297
