| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 40S ribosomal protein S6; Phosphoprotein NP33; Small ribosomal subunit protein eS6; RPS6; OK/SW-cl.2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human RPS6, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of RPS6 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-RPS6 Antibody Picoband® (monoclonal, 2H7) catalog # M01567. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Mouse Monoclonal Mouse IgG1
- Clone number: Clone: 2H7
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human RPS6, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 29 kDa; calculated MW: nan
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.
Biological background
ribosomal protein S6. Ribosomal protein S6 (rpS6) is a component of the 40S ribosomal subunit and is therefore thought to be involved in regulating translation. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. While the true function of rpS6 is currently under investigation, studies have shown that it is involved in the regulation of cell size, cell proliferation, and glucose homeostasis. Functional note: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Reported localization: Cytosol. Cytosolic small ribosomal subunit. Endoplasmic reticulum. Nucleus. Nucleoplasm. Nucleolus. Cell body. Cytoplasmic ribonucleoprotein granule. Dendrite. Membrane. Ribonucleoprotein complex. Small ribosomal subunit.
Research relevance and current trends
- DNA/RNA: Researchers commonly examine how RPS6 relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how RPS6 relates to this theme using model systems and orthogonal readouts.
- Ribosome: Researchers commonly examine how RPS6 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative RPS6 levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of RPS6 across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
