| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Bovine brain S-100b. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1) is an antibody targeting S100B. Common applications include IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: SH-B1; isotype: Mouse IgG1; reactivity: Human,Rabbit,Rat; observed MW: 34 kDa; calculated MW: 10744 MW.
Boster Bio Anti-S-100 (Beta-subunit) S100b Antibody (Monoclonal, SH-B1) catalog # MA1088. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.
Key elements and design rationale
- Target: S100B — Protein S100-B
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: SH-B1; Isotype: Mouse IgG1
- Species reactivity: Human,Rabbit,Rat
- Molecular weight guidance: Observed: 34 kDa; Calculated: 10744 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .
Scientific background (datasheet): S100 protein is a 21,000-Da component first isolated from brain by Moore (1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin (Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a (0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.
Cellular localization (datasheet): Cytoplasm . Nucleus .
Tissue details (datasheet): Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. .
Sequence similarities (datasheet): Belongs to the S-100 family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology,Cell Cycle,Cell Differentiation,Epigenetics and Nuclear Signaling,Neural Stem Cells,Stem Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
