| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein S100-A1;S-100 protein alpha chain;S-100 protein subunit alpha;S100 calcium-binding protein A1;S100A1;S100A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human S100 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-S100 S100A1 Rabbit Monoclonal Antibody is an antibody targeting S100A1. Common applications include WB, IHC, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: ABCF-19; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 34 kDa; calculated MW: 10546 MW.
Boster Bio Anti-S100 S100A1 Rabbit Monoclonal Antibody catalog # M02503-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: S100A1 — Protein S100-A1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: ABCF-19; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 34 kDa; Calculated: 10546 MW
Biological background
Protein function (datasheet): Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .
Cellular localization (datasheet): Cytoplasm.
Tissue details (datasheet): Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Research relevance and current trends
- Commonly studied in contexts related to Calcium Binding Proteins,Calcium Signaling,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Signal Transduction,Signaling Pathway.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
