| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein S100-A10;Calpactin I light chain;Calpactin-1 light chain;Cellular ligand of annexin II;S100 calcium-binding protein A10;p10 protein;p11;S100A10;ANX2LG, CAL1L, CLP11; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human S100A10 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AODO-19 is a rabbit monoclonal antibody that recognizes protein S100-A10 (gene S100A10) in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 11.2 kDa, and the supplier reports an observed band at about 11 kDa.
S100A10 (also called p11) is an S100 family protein that forms a tight heterotetramer with annexin A2 at the inner face of the plasma membrane. Unlike most S100 proteins it does not bind calcium, and the complex is studied in membrane trafficking, plasminogen activation at the cell surface and serotonin receptor signaling.
| Target | Protein S100-A10 (gene S100A10; UniProt P60903, human) |
|---|---|
| Clone | AODO-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.2 kDa |
| Observed MW | 11 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells, RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human S100A10.
Reactivity Notes
The supplier lists reactivity with human and mouse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
