| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sterile alpha motif domain-containing protein 9-like; SAM domain-containing protein 9-like; SAMD9L; C7orf6; DRIF2; KIAA2005; UEF |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SAMD9L recombinant protein (Position: L1223-R1557). Human SAMD9L shares 69.3% amino acid (aa) sequence identity with mouse SAMD9L, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sterile alpha motif domain-containing protein 9-like (gene SAMD9L) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 184.5 kDa, and the supplier reports an observed band at about 200 kDa.
SAMD9L is a sterile alpha motif domain protein that has been linked to endosome fusion and to the internalization and down-regulation of growth factor receptors. It is studied as a regulator of hematopoiesis, and germline SAMD9L variants are associated with inherited bone marrow failure and myeloid disorders.
| Target | Sterile alpha motif domain-containing protein 9-like (gene SAMD9L; UniProt Q8IVG5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 184.5 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U251 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human appendiceal carcinoid tissue, human breast cancer tissue, human cervix squamous cells carcinoma tissue, human placenta tissue, human rectum adenocarcinoma tissue, human spleen tissue, human testicular seminoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human placenta tissue.
Samples with a confirmed signal (flow cytometry): U251 cells.
Immunogen
Recombinant human SAMD9L fragment (Leu1223–Arg1557), expressed in E. coli. Sequence identity with the mouse ortholog: 69.3%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sterile alpha motif domain-containing protein 9-like in the early endosome and mitochondrion. Tissue expression noted by UniProt (human): Widely expressed in adult and fetal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
