Anti-SAMHD1 Antibody Picoband® (monoclonal, 10H8)

Overview

Anti-SAMHD1 Antibody Picoband® (monoclonal, 10H8) is an antibody for SAMHD1 detection raised in Mouse (Monoclonal, clone Clone: 10H8, Mouse IgG2b), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SAMHD1 (EWS RNA-binding protein 1); UniProt: Q9Y3Z3
• Antibody format: Mouse, Monoclonal, clone Clone: 10H8, Mouse IgG2b
• Molecular weight: 72 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-SAMHD1 Antibody Picoband® (monoclonal, 10H8) catalog # M00592-3.

Biological background

Biological context: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Expression and localization notes: cellular localization: Nucleus, tissue context: Ubiquitous..

Common research applications

• Western blotting (WB): Compare SAMHD1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SAMHD1 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify SAMHD1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus
• Tissue details: Ubiquitous.
• Research category: Cancer Susceptibility,Cell Type Markers,Chromatin Binding Proteins,DNA/RNA Binding,Epigenetics and Nuclear Signaling,Proto-Oncogenes,Tags & Cell Markers,Transcription,Transcription Factors,Tumor Associated

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.