| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Disks large homolog 3;Neuroendocrine-DLG;Synapse-associated protein 102;SAP-102;SAP102;XLMR;DLG3;KIAA1232; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human SAP102 recombinant protein (Position: E749-L817). Human SAP102 shares 98.6% amino acid (aa) sequence identity with both mouse and rat SAP102. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of DLG3 (Chromodomain-helicase-DNA-binding protein 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-SAP102/DLG3 Antibody Picoband® catalog # A04152-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human SAP102 recombinant protein (Position: E749-L817). Human SAP102 shares 98.6% amino acid (aa) sequence identity with both mouse and rat SAP102. (reported region: E749-L817).
- Molecular weight context: reported MW: 102 kDa; calculated MW: 90314 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Chromodomain-helicase-DNA-binding protein 2; Disks large homolog 3. Disks large homolog 3 (DLG3) also known as neuroendocrine-DLG or synapse-associated protein 102 (SAP-102) is a protein that in humans is encoded by the DLG3 gene. DLG3 is a member of the membrane-associated guanylate kinase (MAGUK) superfamily of proteins. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked mental retardation. Functional note: Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling. Reported localization: Nucleus speckle. PML body. Nucleus. Expression/tissue context: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
Research relevance and current trends
- Adapters: Researchers commonly examine how DLG3 (Chromodomain-helicase-DNA-binding protein 2) relates to this theme using model systems and orthogonal readouts.
- Cell Type Marker: Researchers commonly examine how DLG3 (Chromodomain-helicase-DNA-binding protein 2) relates to this theme using model systems and orthogonal readouts.
- Intracellular Signaling: Researchers commonly examine how DLG3 (Chromodomain-helicase-DNA-binding protein 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative DLG3 (Chromodomain-helicase-DNA-binding protein 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of DLG3 (Chromodomain-helicase-DNA-binding protein 2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
