| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Small ubiquitin-related modifier 2 ;SUMO-2 ;HSMT3 ;SMT3 homolog 2 ;SUMO-3 ;Sentrin-2 ;Ubiquitin-like protein SMT3B ;Smt3B ;SUMO2 ;SMT3B , SMT3H2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human SAP97 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-DLG1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24D56; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-SAP97 Rabbit Monoclonal Antibody catalog # M01287. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: DLG1 (Small ubiquitin-related modifier 2).
- Antibody format: Monoclonal; clone 24D56; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
DLG1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). . Reported cellular localization context: Nucleus. Nucleus, PML body. Tissue expression notes (as provided): Broadly expressed. .
Research relevance and current trends
- Research context keywords from the source record include: Adaptive Immunity,B Cells,Host-Virus Interaction,Human Protein,Immunology,Interspecies Interaction,Microbiology,Protein.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate DLG1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DLG1 expression by Western blot in cell or tissue lysates, Compare relative DLG1 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 130 kDa; calculated MW: 10871 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 130 kDa
- Cellular localization (provided): Nucleus. Nucleus, PML body.
- Tissue details (provided): Broadly expressed. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
