| Field | Specification |
|---|---|
| Target | |
| Alternative names | Disks large homolog 1; Synapse-associated protein 97; SAP-97; SAP97; hDlg; DLG1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SAP97 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24D56 is a rabbit monoclonal antibody that recognizes disks large homolog 1 (gene DLG1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 100.5 kDa, and the supplier reports an observed band at about 140 kDa.
DLG1 (SAP97) is a membrane-associated guanylate kinase (MAGUK) scaffold protein that clusters receptors, channels and signaling proteins at specific membrane sites. It supports epithelial polarity and cell junctions and also functions at synapses and the immunological synapse. Its Drosophila counterpart, Discs large, is a classic model tumor suppressor, and DLG1 is studied in cell polarity and signaling.
| Target | Disks large homolog 1 (gene DLG1; UniProt Q12959, human) |
|---|---|
| Clone | 24D56 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 100.5 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SAP97.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places disks large homolog 1 in the cell membrane, basolateral cell membrane, endoplasmic reticulum membrane and postsynaptic density. Tissue expression noted by UniProt (human): Abundantly expressed in atrial myocardium (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
