| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-binding protein SATB1; Special AT-rich sequence-binding protein 1; SATB1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human SATB1 recombinant protein (Position: D638-D763). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA-binding protein SATB1 in human, mouse and rat samples and is validated for ELISA, flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 86.0 kDa, and the supplier reports an observed band at about 100 kDa.
SATB1 is a chromatin organizer that binds AT-rich matrix attachment regions and folds DNA into loops, recruiting chromatin-remodeling enzymes, HDACs or HATs to target genes. It is highly expressed in thymocytes, where it shapes T-cell development, and it has also been studied in globin gene switching and cancer progression.
| Target | DNA-binding protein SATB1 (gene SATB1; UniProt Q01826, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 86.0 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human SATB1 fragment (Asp638–Asp763), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA-binding protein SATB1 in the nucleus matrix and nucleus. Tissue expression noted by UniProt (human): Expressed predominantly in thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
H Yi et al. (2022) Expression of SATB1 is a prognostic indicator for survival in diffuse large B-cell lymphoma patients. European Review for Medical and Pharmacological Sciences. 10.26355/eurrev_202201_27862
