| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Lymphocyte antigen 6A-2/6E-1;Ly-6A.2/Ly-6E.1;Stem cell antigen 1;SCA-1;T-cell-activating protein;TAP;Ly6a;Ly6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Ly6a (Lymphocyte antigen 6A-2/6E-1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Sca1/Ly6A/E Antibody Picoband® catalog # A30403. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E.
- Molecular weight context: reported MW: 14 kDa; calculated MW: 14377 MW
- Reactivity: Mouse
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6A-2/6E-1. Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation. Functional note: T-cell activation. Reported localization: Cell membrane; Lipid-anchor, GPI-anchor. Expression/tissue context: Widely expressed. .
Research relevance and current trends
- Chemokines: Researchers commonly examine how Ly6a (Lymphocyte antigen 6A-2/6E-1) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how Ly6a (Lymphocyte antigen 6A-2/6E-1) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how Ly6a (Lymphocyte antigen 6A-2/6E-1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Ly6a (Lymphocyte antigen 6A-2/6E-1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Ly6a (Lymphocyte antigen 6A-2/6E-1) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
