| Field | Specification |
|---|---|
| Target | |
| Alternative names | PH domain leucine-rich repeat-containing protein phosphatase 1; Pleckstrin homology domain-containing family E member 1; PH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein; hSCOP; PHLPP1; KIAA0606; PHLPP; PLEKHE1; SCOP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SCOP/PHLPP1 recombinant protein (Position: R1083-A1436). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes PH domain leucine-rich repeat-containing protein phosphatase 1 (gene PHLPP1) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 184.7 kDa, and the supplier reports an observed band at about 140 kDa.
PHLPP1 (also called SCOP) is a PP2C-family phosphatase that switches off Akt and conventional PKC by removing the phosphate from their hydrophobic-motif serine. By limiting Akt survival signaling and promoting PKC turnover, it tends to favor apoptosis and restrain proliferation, so it is studied as a candidate tumor suppressor and in neuronal signaling and memory.
| Target | PH domain leucine-rich repeat-containing protein phosphatase 1 (gene PHLPP1; UniProt O60346, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 184.7 kDa |
| Observed MW | 140 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human A375 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human SCOP/PHLPP1 fragment (Arg1083–Ala1436), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places PH domain leucine-rich repeat-containing protein phosphatase 1 in the cytoplasm, membrane and nucleus. Tissue expression noted by UniProt (human): In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
