| Field | Specification |
|---|---|
| Target | |
| Alternative names | Synaptonemal complex protein 3;SCP-3;SYCP3;SCP3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SCP3 recombinant protein (Position: E18-E223). Human SCP3 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat SCP3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets synaptonemal complex protein 3 (gene SYCP3) in human samples and is validated for Western blot. The predicted molecular weight is 27.7 kDa, and the supplier reports an observed band at about 28 kDa.
Synaptonemal complex protein 3 (SYCP3, also called SCP3) is a structural component of the lateral elements of the synaptonemal complex that assemble along meiotic chromosome axes. It is required for normal spermatogenesis and male fertility and is widely used as a marker of meiotic prophase in germ cells.
| Target | Synaptonemal complex protein 3 (gene SYCP3; UniProt Q8IZU3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 27.7 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HT1080 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human SCP3 fragment (Glu18–Glu223), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 72% and 73%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places synaptonemal complex protein 3 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Testis-specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
