| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; CPAMD8; KIAA1283 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human SDF2 recombinant protein (Position: K75-L211). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SDF2 Antibody Picoband® is an antibody for SDF2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SDF2 (C3 and PZP like, alpha-2-macroglobulin domain containing 8); UniProt: Q99470
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 23 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-SDF2 Antibody Picoband® catalog # A12906-1.
Biological background
Biological context: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Expression and localization notes: cellular localization: Secreted. Cell membrane. Peripheral membrane protein. Extracellular side., tissue context: Highly expressed in the kidney, brain and testis and to a lower extent in heart, liver and small intestine. Expressed in the lens, cornea and retina. Strongly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body..
Common research applications
- Western blotting (WB): Compare SDF2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SDF2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SDF2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: The protein encoded by this gene is believed to be a secretory protein. It has regions of similarity to hydrophilic segments of yeast mannosyltransferases. Its expression is ubiquitous and the gene appears to be relatively conserved among mammals. Alternate splicing results in both coding and non-coding variants. A pseudogene of this gene is located on chromosome 15.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted. Cell membrane. Peripheral membrane protein. Extracellular side.
- Tissue details: Highly expressed in the kidney, brain and testis and to a lower extent in heart, liver and small intestine. Expressed in the lens, cornea and retina. Strongly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body.
- Research category: Complement,Immunology,Innate Immunity
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
