| Field | Specification |
|---|---|
| Target | |
| Alternative names | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial;1.3.5.1;Flavoprotein subunit of complex II;Fp;SDHA;SDH2, SDHF; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SDHA recombinant protein (Position: S44-L380). Human SDHA shares 98.2% and 97.6% amino acid (aa) sequence identity with mouse and rat SDHA, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes succinate dehydrogenase (gene SDHA) in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 72.7 kDa, and the supplier reports an observed band at about 73 kDa.
SDHA is the flavoprotein subunit of succinate dehydrogenase, the enzyme that is both mitochondrial respiratory complex II and a step in the TCA cycle. It oxidizes succinate to fumarate and passes the electrons on to ubiquinone, and SDHA is studied in mitochondrial disease and as a tumor suppressor in paraganglioma research.
| Target | Succinate dehydrogenase (gene SDHA; UniProt P31040, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 72.7 kDa |
| Observed MW | 73 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells lysate, human MCF-7 cells lysate, human HepG2 cells lysate, human HeLa cells lysate, rat brain tissue lysates, rat kidney tissue lysates, mouse brain tissue lysates, mouse kidney tissue lysates.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human ovarian cancer tissue, mouse heart tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed result (IP): HepG2 cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human SDHA fragment (Ser44–Leu380), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.2% and 97.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places succinate dehydrogenase in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan Liu et al. (2024) Astroglial membrane camouflaged Ptbp1 siRNA delivery hinders glutamate homeostasis via SDH/Nrf2 pathway. Biomaterials. 10.1016/j.biomaterials.2024.122707
Lei Chen et al. (2024) Fucoidan prevent diabetic cognitive dysfunction via promoting TET2-mediated active DNA demethylation in high-fat diet induced diabetic mice. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.134186
Sai Yao et al. (2025) Young Apple Polyphenols Prevent Diabetic Kidney Disease via TET2-Mediated Active DNA Demethylation to Enhance Klotho Expression. Journal of Future Foods. 10.1016/j.jfutfo.2025.09.043
Xin He et al. (2022) Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5-Hydroxymethylcytosine in the Diabetic Mouse Brain. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.2c00863
