Anti-SDHA Antibody Picoband®

Overview

This antibody is intended for detection of SDHA (Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-SDHA Antibody Picoband® catalog # PB9433. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E.coli-derived human SDHA recombinant protein (Position: S44-L380). Human SDHA shares 98.2% and 97.6% amino acid (aa) sequence identity with mouse and rat SDHA, respectively. (reported region: S44-L380).
• Molecular weight context: reported MW: 73 kDa; calculated MW: 72692 MW
• Reactivity: Human,Mouse,Rat
• Applications: Flow Cytometry, IP, IF, IHC, ICC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial. Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit (SDHA), the iron sulfur protein subunit (SDHB), and the integral membrane protein subunits SDHC and SDHD. SDHA is an acronym for succinate dehydrogenase complex subunit A. The succinate dehydrogenase (SDH) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol). The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7 kDA. Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits; it is the only subunit to never have shown tumor suppressor behaviour. Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits. This appears to be due to the expression of two similar SDHA genes (Types I and II) in the paraganglia system. Functional note: Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor. . Reported localization: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Expression/tissue context: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

Research relevance and current trends

• Cancer: Researchers commonly examine how SDHA (Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial) relates to this theme using model systems and orthogonal readouts.
• Cancer Metabolism: Researchers commonly examine how SDHA (Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial) relates to this theme using model systems and orthogonal readouts.
• Energy Transfer Pathways: Researchers commonly examine how SDHA (Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative SDHA (Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of SDHA (Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
• Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.