| Field | Specification |
|---|---|
| Target | |
| Alternative names | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial;1.3.5.1;Iron-sulfur subunit of complex II;Ip;SDHB;SDH, SDH1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SDHB |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABDF-19 is a rabbit monoclonal antibody that recognizes succinate dehydrogenase (gene SDHB) in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 29 kDa.
SDHB is the iron-sulfur subunit of succinate dehydrogenase (mitochondrial complex II), which carries electrons from succinate oxidation toward ubiquinone. Germline SDHB mutations predispose to paragangliomas and pheochromocytomas, and loss of SDHB staining is used in tumor research.
| Target | Succinate dehydrogenase (gene SDHB; UniProt P21912, human) |
|---|---|
| Clone | ABDF-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.6 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human Jurkat cells, human HepG2 cells, human HeLa- WT cells, human HeLa-SDHB KO cells, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human SDHB.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places succinate dehydrogenase in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
