| Field | Specification |
|---|---|
| Target | |
| Alternative names | Semaphorin-3A;Semaphorin III;Sema III;SEMA3A;SEMAD; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Semaphorin 3A recombinant protein (Position: K550-V771). Human Semaphorin 3A shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Semaphorin 3A, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes semaphorin-3A (gene SEMA3A) in human samples and is validated for Western blot. The predicted molecular weight is 88.9 kDa, and the supplier reports an observed band at about 89 kDa.
Semaphorin 3A (SEMA3A) is a secreted guidance protein that binds neuropilin-1 and plexin-A co-receptors. It causes growth cone collapse and repels growing axons, shaping neural wiring, and it also affects blood vessel formation, bone homeostasis and immune responses.
| Target | Semaphorin-3A (gene SEMA3A; UniProt Q14563, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 88.9 kDa |
| Observed MW | 89 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): 293T cells, A549 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human Semaphorin 3A fragment (Lys550–Val771), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93% and 94%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes semaphorin-3A as a secreted protein. Tissue expression noted by UniProt (human): Expressed in the dorsal root ganglia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lingjuan Du et al. (2018) Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A. International Journal of Clinical and Experimental Pathology. PMID: 31949563
Haiying Zhou et al. (2014) Significance of semaphorin‑3A and MMP‑14 protein expression in non‑small cell lung cancer. Oncology Letters. 10.3892/ol.2014.1920
