Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7)

Overview

Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7) is an antibody for SEMA3B detection raised in Mouse (Monoclonal, clone Clone: 9C4F7, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SEMA3B (chaperonin containing TCP1 subunit 3); UniProt: Q13214
• Antibody format: Mouse, Monoclonal, clone Clone: 9C4F7, Mouse IgG2b
• Molecular weight: 83 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Semaphorin 3B/SEMA3B Antibody Picoband® (monoclonal, 9C4F7) catalog # M06559.

Biological background

Biological context: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Expression and localization notes: cellular localization: Cytoplasm., tissue context: Ubiquitously expressed with highest levels in spleen, thymus and immature brain..

Common research applications

• Western blotting (WB): Compare SEMA3B levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SEMA3B in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify SEMA3B-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Semaphorin-3Bis aproteinthat in humans is encoded by theSEMA3Bgene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm.
• Tissue details: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
• Research category: Chaperones,Protein Trafficking,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.