| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sentrin-specific protease 1; Sentrin/SUMO-specific protease SENP1; SENP1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SENP1 recombinant protein (Position: N19-P619). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sentrin-specific protease 1 (gene SENP1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 73.5 kDa, and the supplier reports an observed band at about 73 kDa.
SENP1 is a SUMO-specific cysteine protease with two jobs: it trims SUMO1, SUMO2 and SUMO3 precursors into their mature forms, and it removes SUMO from modified proteins. By reversing SUMOylation it controls the activity and localization of many nuclear proteins, including transcription factors, and is studied in gene regulation, hypoxia signaling and cancer biology.
| Target | Sentrin-specific protease 1 (gene SENP1; UniProt Q9P0U3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 73.5 kDa |
| Observed MW | 73 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells, human HepG2 cells, human HeLa cells, human Jurkat cells, human U87 cells, human Raji cells, human HL-60 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human SENP1 fragment (Asn19–Pro619), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sentrin-specific protease 1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
