| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serpin B3; Protein T4-A; Squamous cell carcinoma antigen 1; SCCA-1; SERPINB3; SCCA; SCCA1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from SerpinB3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26S64 is a rabbit monoclonal antibody that recognizes serpin B3 (gene SERPINB3) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 44.6 kDa, and the supplier reports an observed band at about 39 kDa.
Serpin B3 (SERPINB3, also known as squamous cell carcinoma antigen 1, SCCA1) is an intracellular serpin that inhibits papain-like cysteine proteases rather than serine proteases. It can protect cells from UV-induced apoptosis by suppressing JNK1 activity and is widely studied in squamous epithelia and squamous cell carcinoma.
| Target | Serpin B3 (gene SERPINB3; UniProt P29508, human) |
|---|---|
| Clone | 26S64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.6 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of serpinB3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serpin B3 in the cytoplasm. Tissue expression noted by UniProt (human): Squamous cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
