| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone-lysine N-methyltransferase SETD7;2.1.1.43;Histone H3-K4 methyltransferase SETD7;H3-K4-HMTase SETD7;Lysine N-methyltransferase 7;SET domain-containing protein 7;SET7/9;SETD7;KIAA1717, KMT7, SET7, SET9; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SETD7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABA-19 is a rabbit monoclonal antibody that recognizes histone-lysine N-methyltransferase SETD7 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 40.7 kDa, and the supplier reports an observed band at about 49 kDa.
SETD7 (also called SET7/9) is a methyltransferase that adds a single methyl group to histone H3 at lysine 4, a mark linked to transcriptional activation. It also methylates many non-histone proteins, including p53, which alters their stability or activity, so it is studied in gene regulation, metabolism and cancer.
| Target | Histone-lysine N-methyltransferase SETD7 (gene SETD7; UniProt Q8WTS6, human) |
|---|---|
| Clone | ABA-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.7 kDa |
| Observed MW | 49 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): Jurkat cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SETD7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places histone-lysine N-methyltransferase SETD7 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
