| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/arginine-rich splicing factor 1; Alternative-splicing factor 1; ASF-1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit; SRSF1; ASF; SF2; SF2P33; SFRS1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SF2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADGH-19 is a rabbit monoclonal antibody that recognizes serine/arginine-rich splicing factor 1 (gene SRSF1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 27.7 kDa, and the supplier reports an observed band at about 33 kDa.
Serine/arginine-rich splicing factor 1 (SRSF1, also called SF2/ASF) is a prototype SR protein that binds purine-rich exonic enhancers. It helps recruit U1 snRNP and U2AF to splice sites, prevents exon skipping and regulates alternative splicing, and it also functions in mRNA export and translation, so it is widely studied in RNA processing and cancer.
| Target | Serine/arginine-rich splicing factor 1 (gene SRSF1; UniProt Q07955, human) |
|---|---|
| Clone | ADGH-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.7 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): mouse spleen.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SF2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/arginine-rich splicing factor 1 in the chromosome, cytoplasm and nucleus speckle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Han Zhou et al. (2025) CircGNAQ Promotes Intracranial Aneurysm Formation by Facilitating Vascular Smooth Muscle Cell Phenotypic Switching and Apoptosis. Antioxidants & Redox Signaling. 10.1177/15230864251380271
