| Field | Specification |
|---|---|
| Target | |
| Alternative names | Splicing factor 3A subunit 1; SF3a120; Spliceosome-associated protein 114; SAP 114; SF3A1; SAP114 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SF3A1 recombinant protein (Position: K20-Q556). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets splicing factor 3A subunit 1 (gene SF3A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and ELISA. The predicted molecular weight is 88.9 kDa, and the supplier reports an observed band at about 120–130 kDa.
Splicing factor 3A subunit 1 (SF3A1, SF3a120) is a component of the SF3a complex within the U2 snRNP of the spliceosome. SF3a helps the U2 snRNP engage the intron branch site and select the branch-point adenosine during early spliceosome assembly, so SF3A1 is studied in pre-mRNA splicing.
| Target | Splicing factor 3A subunit 1 (gene SF3A1; UniProt Q15459, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 88.9 kDa |
| Observed MW | 120–130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human K562 cells, human 293T cells, human U251 cells, human Daudi cells, human MOLT-4 cells, rat lung tissue, rat brain tissue, rat thymus tissue, rat C6 cells, mouse lung tissue, mouse brain tissue, mouse thymus tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human spleen tissue, human larynx squamous cells carcinoma tissue, human liver cancer tissue, human lung adenocarcinoma tissue, human ovarian serous cancer tissue, human prostate adenocarcinoma tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue, rat colon tissue.
Samples with a confirmed result (IP): HeLa cells.
Immunogen
Recombinant human SF3A1 fragment (Lys20–Gln556), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places splicing factor 3A subunit 1 in the nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
