| Field | Specification |
|---|---|
| Target | |
| Alternative names | Secreted frizzled-related protein 2;FRP-2;sFRP-2;Secreted apoptosis-related protein 1;SARP-1;SFRP2;FRP2, SARP1;FKSG12, UNQ361/PRO697; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SFRP2 recombinant protein (Position: L104-C295). Human SFRP2 shares 99% amino acid (aa) sequence identity with mouse SFRP2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets secreted frizzled-related protein 2 (gene SFRP2) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 33.5 kDa, and the supplier reports an observed band at about 33 kDa.
Secreted frizzled-related protein 2 (SFRP2) is a soluble Wnt-binding protein that modulates Wnt signaling by interacting directly with Wnt ligands. It influences cell growth and differentiation in specific tissues and may be important for retinal development and muscle formation.
| Target | Secreted frizzled-related protein 2 (gene SFRP2; UniProt Q96HF1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 33.5 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): COLO320 cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human SFRP2 fragment (Leu104–Cys295), expressed in E. coli. Sequence identity with the mouse ortholog: 99%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes secreted frizzled-related protein 2 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
