Anti-SFTPA1/2 Antibody Picoband®

Overview

This antibody is intended for detection of SFTPA2 (Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-SFTPA1/2 Antibody Picoband® catalog # PB9390. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPA1/2, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
• Molecular weight context: reported MW: 26 kDa; calculated MW: 26182 MW
• Reactivity: Human,Mouse,Rat
• Applications: IF, IHC, IHC-F, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2; Pulmonary surfactant-associated protein A2. SFTPA1/2 is also known as SP-A. SFTPA1 encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants. SFTPA2 is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication. Functional note: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Reported localization: Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. Expression/tissue context: Expressed in B-cell lines and T-lymphocytes. .

Research relevance and current trends

• Cytoskeleton/ECM: Researchers commonly examine how SFTPA2 (Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2) relates to this theme using model systems and orthogonal readouts.
• ECM Proteins: Researchers commonly examine how SFTPA2 (Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2) relates to this theme using model systems and orthogonal readouts.
• Extracellular Matrix: Researchers commonly examine how SFTPA2 (Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative SFTPA2 (Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of SFTPA2 (Pulmonary surfactant-associated protein A1/Pulmonary surfactant-associated protein A2) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Family / similarity context: Belongs to the SFTPA family.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.