| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1; SGK1; SGK |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SGK1, which shares 91.3% and 95.7% amino acid (aa) sequence identity with mouse and rat SGK1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects serine/threonine-protein kinase Sgk1 in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 48.9 kDa, and the supplier reports an observed band at about 55 kDa.
Serum- and glucocorticoid-regulated kinase 1 (SGK1) is a serine/threonine kinase of the AGC family whose expression is rapidly induced by hormones and cell stress. Activated downstream of PI3K through mTORC2 and PDK1, it regulates many ion channels and transporters, notably the epithelial sodium channel in the kidney, and also affects cell survival and proliferation.
| Target | Serine/threonine-protein kinase Sgk1 (gene SGK1; UniProt O00141, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 48.9 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 4 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human placenta tissue, human A431 cells, human PANC-1 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Synthetic peptide from the N-terminal region of human SGK1. Sequence identity with the mouse and rat orthologs: 91.3% and 95.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine/threonine-protein kinase Sgk1 in the cytoplasm, nucleus, endoplasmic reticulum membrane and cell membrane. Tissue expression noted by UniProt (human): Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
