| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1;3.1.3.86;Inositol polyphosphate-5-phosphatase of 145 kDa;SIP-145;SH2 domain-containing inositol 5'-phosphatase 1;SH2 domain-containing inositol phosphatase 1;SHIP-1;p150Ship;hp51CN;INPP5D;SHIP, SHIP1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SHIP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AHG-9 is a rabbit monoclonal antibody that recognizes phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (gene INPP5D) in human samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 133.3 kDa, and the supplier reports an observed band at about 140 kDa.
SHIP1 (encoded by INPP5D) is an SH2 domain-containing inositol 5-phosphatase expressed mainly in blood cells. It converts PI(3,4,5)P3 to PI(3,4)P2, acting as a negative regulator of PI3K signaling downstream of immune receptors. SHIP1 limits activation of B cells, mast cells and myeloid cells and is studied in immunology and microglial biology.
| Target | Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (gene INPP5D; UniProt Q92835, human) |
|---|---|
| Clone | AHG-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 133.3 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): Daudi cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human SHIP.
Reactivity Notes
The supplier lists reactivity with human. UniProt places phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 in the cytoplasm, cell membrane, membrane raft and membrane. Tissue expression noted by UniProt (human): Specifically expressed in immune and hematopoietic cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
