Anti-SHISA6 Antibody Picoband®

Overview

Anti-SHISA6 Antibody Picoband® is an antibody for SHISA6 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SHISA6 (Transmembrane protein 240); UniProt: Q6ZSJ9
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 56 kDa, calculated 19908 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-SHISA6 Antibody Picoband® catalog # A14975-1.

Biological background

Biological context: Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Expression and localization notes: cellular localization: Cell junction, synapse . Cell membrane ; Multi-pass membrane protein ., tissue context: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon..

Common research applications

• Western blotting (WB): Compare SHISA6 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SHISA6 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify SHISA6-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The deduced 551-amino acid human SHISA6 protein has a domain structure typical of SHISA proteins, including an N-terminal signal peptide, followed by a cysteine-rich domain, a transmembrane domain, and a C-terminal proline-rich region. SHISA6 belongs to a SHISA subfamily containing SHISA7, SHISA8, and SHISA9. The SHISA6 gene is mapped to chromosome 17p12 based on an alignment of the SHISA6 sequence (GenBank AK127379) with the genomic sequence (GRCh38).
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell junction, synapse . Cell membrane ; Multi-pass membrane protein .
• Tissue details: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
• Research category: Cell Biology

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.