| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sucrase-isomaltase, intestinal; Sucrase; Isomaltase; SI |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SI, which shares 86.1% amino acid (aa) sequence identity with rat SI. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sucrase-isomaltase, intestinal (gene SI) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 209.5 kDa, and the supplier reports an observed band at about 209 kDa.
Sucrase-isomaltase (SI) is a brush-border enzyme of the small intestine with two catalytic domains. The sucrase domain splits sucrose into glucose and fructose and also cleaves maltose, while the isomaltase domain breaks alpha-1,6 bonds in starch-derived oligosaccharides; inherited deficiency causes congenital sucrase-isomaltase deficiency.
| Target | Sucrase-isomaltase, intestinal (gene SI; UniProt P14410, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 209.5 kDa |
| Observed MW | 209 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): rat small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse small intestine tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Synthetic peptide from the N-terminal region of human SI. Sequence identity with the rat ortholog: 86.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sucrase-isomaltase, intestinal in the apical cell membrane. Tissue expression noted by UniProt (human): Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Runyu et al. (2024) Potential hypoglycemic activity of anthocyanidins monoglucoside and diglucoside from Yan 73 (Vitis vinifera L.) and spine grape (Vitis davidii Foex)—effect on α-glucosidases of gut microbiota and human Caco-2 cells. European Food Research and Technology. 10.1007/s00217-024-04524-w
