| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sigma non-opioid intracellular receptor 1; Aging-associated gene 8 protein; SR31747-binding protein; SR-BP; Sigma 1-type opioid receptor; SIG-1R; Sigma1-receptor; Sigma1R; hSigmaR1; SIGMAR1; OPRS1; SRBP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Sigma1-receptor/SIGMAR1 recombinant protein (Position: G1-H154). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes sigma non-opioid intracellular receptor 1 (gene SIGMAR1) in human, monkey, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 25.1 kDa, and the supplier reports an observed band at about 25 kDa.
The sigma-1 receptor (SIGMAR1) is a ligand-regulated chaperone located mainly at the endoplasmic reticulum membrane contacting mitochondria. It modulates IP3 receptor-mediated Ca2+ release, ion channels and lipid transport, and because it binds many psychoactive and neuroprotective compounds, it is widely studied in neuroscience and neurodegeneration research.
| Target | Sigma non-opioid intracellular receptor 1 (gene SIGMAR1; UniProt Q99720, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 25.1 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Monkey, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells, human 293T cells, human HepG2 cells, human A549 cells, human COLO-320 cells, human U-87MG cells, monkey COS-7 cells, mouse liver tissue, mouse C2C12 cells, mouse Hepa1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat colon tissue, mouse colon tissue, human adenocarcinoma of the right colon tissue, human stomach cancer tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Sigma1-receptor/SIGMAR1 fragment (Gly1–His154), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places sigma non-opioid intracellular receptor 1 in the nucleus inner membrane, nucleus outer membrane, nucleus envelope and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
