Anti-Sigma1-receptor/SIGMAR1 Antibody Picoband®

Overview

Anti-Sigma1-receptor/SIGMAR1 Antibody Picoband® is an antibody for SIGMAR1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SIGMAR1 (Heat shock protein beta-8); UniProt: Q99720
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 25 kDa, calculated 21604 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Sigma1-receptor/SIGMAR1 Antibody Picoband® catalog # A02493-2.

Biological background

Biological context: Displays temperature-dependent chaperone activity.

Expression and localization notes: cellular localization: Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock., tissue context: Predominantly expressed in skeletal muscle and heart. ..

Common research applications

• Western blotting (WB): Compare SIGMAR1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SIGMAR1 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify SIGMAR1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The sigma-1 receptor (σ1R), one of two sigma receptor subtypes, is a chaperone protein at the endoplasmic reticulum (ER) that modulates calcium signaling through the IP3 receptor. In humans, the σ1 receptor is encoded by the SIGMAR1 gene. This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms.
• Cross reactivity: No cross-reactivity with other proteins
• Cellular localization: Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.
• Tissue details: Predominantly expressed in skeletal muscle and heart. .
• Research category: Chaperones,Heat Shock Proteins,Neurodegenerative Disease,Neurology Process,Neuroscience,Protein Trafficking,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.