| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD-dependent protein deacetylase sirtuin-3, mitochondrial; hSIRT3; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; SIRT3; SIR2L3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SIRT3, which shares 80% amino acid (aa) sequence identity with mouse and rat SIRT3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets NAD-dependent protein deacetylase sirtuin-3, mitochondrial (gene SIRT3) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 43.6 kDa, and the supplier reports an observed band at about 29 kDa.
Sirtuin 3 (SIRT3) is the major NAD+-dependent deacetylase of the mitochondrial matrix. It activates many metabolic enzymes, including SOD2, isocitrate dehydrogenase and components of the electron transport chain, helping mitochondria adapt to fasting and oxidative stress, and it is widely studied in energy metabolism and aging.
| Target | NAD-dependent protein deacetylase sirtuin-3, mitochondrial (gene SIRT3; UniProt Q9NTG7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 43.6 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human 293T cells, human HepG2 cells, human HeLa cells, human HEK293-WT cells, human HEK293-SIRT3 KO cells, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): SiHa cells.
Immunogen
Synthetic peptide from the middle region of human SIRT3. Sequence identity with the mouse and rat orthologs: 80%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NAD-dependent protein deacetylase sirtuin-3, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
