| Field | Specification |
|---|---|
| Target | |
| Alternative names | NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4; SIRT4; SIR2L4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SIRT4, which shares 93.1% amino acid (aa) sequence identity with both mouse and rat SIRT4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes NAD-dependent protein lipoamidase sirtuin-4, mitochondrial (gene SIRT4) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 35.2 kDa, and the supplier reports an observed band at about 38 kDa.
Sirtuin 4 (SIRT4) is a mitochondrial NAD+-dependent enzyme with weak deacetylase activity but notable lipoamidase and ADP-ribosyltransferase activities. It inhibits glutamate dehydrogenase (GLUD1) and the pyruvate dehydrogenase complex, restraining glutamine and glucose oxidation, and it is studied in insulin secretion, fatty acid oxidation and tumor suppression.
| Target | NAD-dependent protein lipoamidase sirtuin-4, mitochondrial (gene SIRT4; UniProt Q9Y6E7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 35.2 kDa |
| Observed MW | 38 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, rat heart tissue, rat liver tissue, mouse brain tissue, mouse heart tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human ovary cancer tissue, human placenta tissue, human gastric cancer tissue, human glioma tissue, human rectal cancer tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human SIRT4. Sequence identity with the mouse and rat orthologs: 93.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NAD-dependent protein lipoamidase sirtuin-4, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Detected in vascular smooth muscle and striated muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shuting Chang et al. (2023) Sirt4 deficiency promotes the development of atherosclerosis by activating the NF-κB/IκB/CXCL2/3 pathway. Atherosclerosis. 10.1016/j.atherosclerosis.2023.04.006
