| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NAD-dependent protein deacylase sirtuin-5, mitochondrial ;3.5.1.- ;Regulatory protein SIR2 homolog 5 ;SIR2-like protein 5 ;SIRT5 ;SIR2L5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human SIRT5 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SIRT5/Sirtuin 5 Rabbit Monoclonal Antibody is an antibody targeting SIRT5. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HHE-19; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 60 kDa; calculated MW: 33881 MW.
Boster Bio Anti-SIRT5/Sirtuin 5 Rabbit Monoclonal Antibody catalog # M02395. Tested in WB application. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: SIRT5 — NAD-dependent protein deacylase sirtuin-5, mitochondrial
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HHE-19; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 60 kDa; Calculated: 33881 MW
Biological background
Protein function (datasheet): NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX. .
Cellular localization (datasheet): Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus.
Tissue details (datasheet): Widely expressed. .
Research relevance and current trends
- Commonly studied in contexts related to Acetylation,Cancer,Cardiovascular,Chromatin Modifying Enzymes,Epigenetics and Nuclear Signaling,Metabolism.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
