| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NAD-dependent protein deacetylase sirtuin-6;3.5.1.-;Regulatory protein SIR2 homolog 6;SIR2-like protein 6;SIRT6;SIR2L6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human SIRT6 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SIRT6 Rabbit Monoclonal Antibody is an antibody targeting SIRT6. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HBA-19; isotype: Rabbit IgG; reactivity: Human; observed MW: 32-42 kDa, 65 kDa, 75 kDa; calculated MW: 39119 MW.
Boster Bio Anti-SIRT6 Rabbit Monoclonal Antibody catalog # M00611. Tested in WB application. This antibody reacts with Human.
Key elements and design rationale
- Target: SIRT6 — NAD-dependent protein deacetylase sirtuin-6
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HBA-19; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 32-42 kDa, 65 kDa, 75 kDa; Calculated: 39119 MW
Biological background
Protein function (datasheet): NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD (+) in the absence of acetylated substrate. .
Cellular localization (datasheet): Nucleus, nucleoplasm . Predominantly nuclear. Associated with telomeric heterochromatin regions.
Tissue details (datasheet): Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. .
Research relevance and current trends
- Commonly studied in contexts related to Acetylation,Apoptosis,Atherosclerosis,Cancer,Cardiovascular,2339,Chromatin Modifying Enzymes,Diabetes-associated,Epigenetics and Nuclear Signaling,Metabolism,NFKB Pathway,Nuclear Signaling Pathways,Obesity,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
