| Field | Specification |
|---|---|
| Target | |
| Alternative names | Signaling lymphocytic activation molecule; CDw150; IPO-3; SLAM family member 1; CD antigen CD150; SLAMF1; SLAM |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLAM/CD150/SLAMF1 recombinant protein (Position: K34-N213). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets signaling lymphocytic activation molecule (gene SLAMF1) in human samples and is validated for IHC, flow cytometry and ELISA. The predicted molecular weight is 37.2 kDa.
SLAMF1 (SLAM, CD150) is a self-binding receptor of the SLAM family found on activated T and B cells, dendritic cells and some other immune cells. Its signaling depends on the adaptors SAP (SH2D1A) and EAT-2, it shapes T-cell cytokine responses and macrophage function, and it also serves as the cellular receptor for measles virus.
| Target | Signaling lymphocytic activation molecule (gene SLAMF1; UniProt Q13291, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.2 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human SLAM/CD150/SLAMF1 fragment (Lys34–Asn213), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places signaling lymphocytic activation molecule in the cell membrane. Tissue expression noted by UniProt (human): Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
