| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Meiotic recombination protein REC8 homolog; Cohesin Rec8p; REC8; REC8L1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SCEL, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SLC14A1/UTE Antibody Picoband® is an antibody for Slc14a1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Slc14a1 (REC8 meiotic recombination protein); UniProt: Q8VHL0
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 48-50 kDa, calculated 56903 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-SLC14A1/UTE Antibody Picoband® catalog # A04926-1.
Biological background
Biological context: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
Expression and localization notes: cellular localization: Nucleus. Chromosome. Centromere., tissue context: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa..
Common research applications
- Western blotting (WB): Compare Slc14a1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Slc14a1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Slc14a1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Urea transporter 1 is a protein that in humans is encoded by the SLC14A1 gene. The SLC14A1 codes for a urea transporter (UTB) that is expressed in erythrocytes and kidney. SLC14A2 and SLC14A1 constitute solute carrier family 14. UTB proteins constitute the Kidd antigen system.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus. Chromosome. Centromere.
- Tissue details: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.
- Research category: Vasculature
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
