| Field | Specification |
|---|---|
| Target | |
| Alternative names | Diablo homolog, mitochondrial; IAP-binding protein with low pI;Second mitochondria-derived activator of caspase;Smac;DIABLO;SMAC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes diablo IAP-binding mitochondrial protein in human, mouse and rat samples and is validated for ELISA, flow cytometry, immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 27.1 kDa, and the supplier reports an observed band at about 20 kDa.
DIABLO (also called Smac) is a mitochondrial protein released into the cytosol during apoptosis. It binds inhibitor of apoptosis proteins such as XIAP and blocks their ability to restrain caspases, thereby promoting caspase activation downstream of cytochrome c. Smac mimetic compounds are based on its IAP-binding motif, and DIABLO is studied in apoptosis research.
| Target | Diablo IAP-binding mitochondrial protein (gene DIABLO; UniProt Q9NR28, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 27.1 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human HepG2 cells, humna Jurkat cells, rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed result (IP): HepG2 cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human Smac/Diablo fragment (Ala56–Asp239), expressed in E. coli. Sequence identity with the mouse ortholog: 88.6%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places diablo IAP-binding mitochondrial protein in the mitochondrion and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed with highest expression in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jie Yu et al. (2013) Effect of moxibustion treatment on cell apoptosis and expressions of heat shock protein and second mitochondrial activator of caspase in acute gastric mucosal lesion of rats. Journal of Traditional Chinese Medicine. 10.1016/S0254-6272(13)60136-3
