| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Zinc finger E-box-binding homeobox 2;Smad-interacting protein 1;SMADIP1;Zinc finger homeobox protein 1b;ZEB2;KIAA0569, SIP1, ZFHX1B, ZFX1B;HRIHFB2411; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ZEB2 (Zinc finger E-box-binding homeobox 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® catalog # PB9236. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2. (reported region: M1-P200).
- Molecular weight context: reported MW: 200 kDa; calculated MW: 136447 MW
- Reactivity: Human
- Applications: Flow Cytometry, IHC, IHC-F, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Zinc finger E-box-binding homeobox 2; Zinc finger E-box-binding homeobox 2. ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition. Functional note: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. . Reported localization: Nucleus . Expression/tissue context: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. .
Research relevance and current trends
- Cancer: Researchers commonly examine how ZEB2 (Zinc finger E-box-binding homeobox 2) relates to this theme using model systems and orthogonal readouts.
- Developmental Biology: Researchers commonly examine how ZEB2 (Zinc finger E-box-binding homeobox 2) relates to this theme using model systems and orthogonal readouts.
- Developmental Families: Researchers commonly examine how ZEB2 (Zinc finger E-box-binding homeobox 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ZEB2 (Zinc finger E-box-binding homeobox 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ZEB2 (Zinc finger E-box-binding homeobox 2) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
