| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc finger E-box-binding homeobox 2;Smad-interacting protein 1;SMADIP1;Zinc finger homeobox protein 1b;ZEB2;KIAA0569, SIP1, ZFHX1B, ZFX1B;HRIHFB2411; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ZEB2, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects zinc finger E-box-binding homeobox 2 (gene ZEB2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 136.4 kDa, and the supplier reports an observed band at about 180 kDa.
ZEB2 (also called SIP1) is a zinc finger E-box-binding homeobox transcription factor that interacts with activated SMAD proteins. It represses E-cadherin and other target genes to promote epithelial–mesenchymal transition, is important in neural crest and nervous system development, and mutations cause Mowat-Wilson syndrome.
| Target | Zinc finger E-box-binding homeobox 2 (gene ZEB2; UniProt O60315, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 136.4 kDa |
| Observed MW | 180 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HEL cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human glioma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human ZEB2. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places zinc finger E-box-binding homeobox 2 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yue Wang et al. (2022) The effects of endosulfan on cell migration and invasion in prostate cancer cells via the KCNQ1OT1/miR-137-3p/PTP4A3 axis. Science of the Total Environment. 10.1016/j.scitotenv.2022.157252
Boxiang Zhang et al. (2022) Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells. Toxicology. 10.1016/j.tox.2022.153392
Yang Zhihao et al. (2023) Integrative analysis of a novel super-enhancer-associated lncRNA prognostic signature and identifying LINC00945 in aggravating glioma progression. Human Genomics. 10.1186/s40246-023-00480-w
Wei Ping et al. (2017) MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by targeting GAS7. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.12.096
Shuang Zhao et al. (2019) Placental growth factor gene silencing mitigates the epithelial‑to‑mesenchymal transition via the p38 MAPK pathway in rats with hyperoxia‑induced lung injury. Molecular Medicine Reports. 10.3892/mmr.2019.10785
