| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes SMAD family member 2 (gene SMAD2) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 52.3 kDa, and the supplier reports an observed band at about 60 kDa.
SMAD2 is a receptor-regulated SMAD that carries signals from TGF-β and activin receptors. After phosphorylation it forms a complex with SMAD4 that enters the nucleus to control gene transcription, so phospho-SMAD2 is a standard readout of TGF-β pathway activity in development, fibrosis and cancer.
| Target | SMAD family member 2 (gene SMAD2; UniProt Q15796, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 52.3 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human MDA-MB-453 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide from the N-terminal region of human smad2. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human. UniProt places SMAD family member 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yue Wang et al. (2022) The effects of endosulfan on cell migration and invasion in prostate cancer cells via the KCNQ1OT1/miR-137-3p/PTP4A3 axis. Science of the Total Environment. 10.1016/j.scitotenv.2022.157252
Yue Wang et al. (2020) Endosulfan triggers epithelial-mesenchymal transition via PTP4A3-mediated TGF-β signaling pathway in prostate cancer cells. Science of the Total Environment. 10.1016/j.scitotenv.2020.139234
Juanping Zhao et al. (2024) Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1. Bioorganic Chemistry. 10.1016/j.bioorg.2024.107274
Wuhao Yang et al. (2024) Co-Mn Complex Oxide Nanoparticles as Potential Reactive Oxygen Species Scavenging Agents for Pulmonary Fibrosis Treatment. Molecules. 10.3390/molecules29215106
Yongchang Guan et al. (2016) Gremlin1 promotes carcinogenesis of glioma in vitro. Clinical and Experimental Pharmacology and Physiology. 10.1111/1440-1681.12697
Kang Yang et al. (2020) Hirudin Ameliorates Renal Interstitial Fibrosis via Regulating TGF-β1/Smad and NF-κB Signaling in UUO Rat Model. Evidence-based Complementary and Alternative Medicine. 10.1155/2020/7291075
