| Field | Specification |
|---|---|
| Target | |
| Alternative names | SMAD family member 2; SMAD 2; hSMAD2; JV18-1; Mad-related protein 2; hMAD-2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; SMAD2; MADH2; MADR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SMAD2 recombinant protein (Position: E83-Q264). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 3C4 is a mouse monoclonal antibody that recognizes SMAD family member 2 (gene SMAD2) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 52.3 kDa, and the supplier reports an observed band at about 55 kDa.
SMAD2 is a receptor-regulated SMAD that carries signals from TGF-β and activin receptors. After phosphorylation it forms a complex with SMAD4 that enters the nucleus to control gene transcription, so phospho-SMAD2 is a standard readout of TGF-β pathway activity in development, fibrosis and cancer.
| Target | SMAD family member 2 (gene SMAD2; UniProt Q15796, human) |
|---|---|
| Clone | 3C4 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.3 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HL-60 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human gallbladder adenocarcinoma tissue, human low-differentiated adenocarcinoma of the intestine tissue, human pancreatic cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human SMAD2 fragment (Glu83–Gln264), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places SMAD family member 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
