| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | JV15-2, HsT17436 |
| Clonality | |
| Concentration | |
| Formulation | |
| Immunogen | The antibody was produced against synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 208 (N-L-SP-P-N). |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Storage |
Product Overview
Research Use OnlyThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
Validated Applications
| Immunohistochemistry (IHC) | 1:50~1:100 IF:1:100~1:200 |
|---|---|
| Immunoprecipitation (IP) | See datasheet for recommended dilution |
Key Specifications
| Clonality | Polyclonal |
|---|---|
| Species Reactivity | Human,Mouse,Rat |
| Isotype | IgG |
| Concentration | 100ug/100ul |
| Formulation | Liquid |
| Catalog No. | Y021324 |
| Alternative Names | JV15-2, HsT17436 |
Immunogen / Antigen
The antibody was produced against synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 208 (N-L-SP-P-N).
Reactivity Notes
Smad3 (Ab-208) Antibody detects endogenous levels of total Smad3 protein.
Additional Information
Application Notes: IHC: 1:50~1:100 IF:1:100~1:200
Storage & Handling
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Immunohistochemistry, Immunoprecipitation. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
IHC: 1:50~1:100 IF:1:100~1:200
This antibody has been confirmed reactive against: Human,Mouse,Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
The antibody was produced against synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 208 (N-L-SP-P-N).
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
